1ILE
ISOLEUCYL-TRNA SYNTHETASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05 |
Detector | RIGAKU |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.100, 94.800, 126.500 |
Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.29800 |
Structure solution method | MIR |
RMSD bond length | 0.017 |
RMSD bond angle | 1.650 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.064 | |
Total number of observations | 134577 * | |
Number of reflections | 48290 | |
<I/σ(I)> | 8.14 | |
Completeness [%] | 91.0 | 76.3 |
Redundancy | 3.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 12 (%) | |
3 | 1 | reservoir | isopropanol | 6 (%) | |
4 | 1 | reservoir | MPD | 1 (%) | |
5 | 1 | reservoir | HEPES | 60 (mM) |