1IKN
IKAPPABALPHA/NF-KAPPAB COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.500, 49.300, 120.600 |
| Unit cell angles | 90.00, 108.70, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.223 * |
| Rwork | 0.223 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.043 | 0.220 |
| Total number of observations | 160828 * | |
| Number of reflections | 28014 | |
| <I/σ(I)> | 15.1 | 2.5 |
| Completeness [%] | 91.0 | 59 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 23-24 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | MES | 25 (mM) | |
| 3 | 1 | drop | PEG8000 | 6.5 (%) | |
| 4 | 1 | drop | dithiothreitol | 2.5 (mM) | |
| 5 | 1 | reservoir | MES | 50 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 10 (%) | |
| 7 | 1 | reservoir | dithiothreitol | 5 (mM) |
