1IJE
Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.712, 90.418, 92.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.400 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f60 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.205 |
| Number of reflections | 21223 | |
| <I/σ(I)> | 22.1 | |
| Completeness [%] | 100.0 | 99 * |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 * | 277 | Pedersen, L.P., (2000) Acta Crystallogr., D57, 159. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 8 | 1 | reservoir | PEG2000 MME | 15-18 (%) |
