1IIY
Solution NMR Structure of Complex of 1:2 Cyanovirin-N:Man-Alpha1,2-Man-Alpha Restrained Regularized Mean Coordinates
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | DMX500 | 500 | |
| 2 | Bruker | DMX600 | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN: CBCA(CO)NH |
| 2 | 1 | 1 | CBCANH |
| 3 | 1 | 1 | HCCH-COSY |
| 4 | 1 | 1 | HCCH-TOCSY |
| 5 | 1 | 1 | 15N-SEPARATED HOHAHA |
| 6 | 1 | 1 | QUANTITATIVE J CORRELATION FOR COUPLING CONSTANTS |
| 7 | 1 | 1 | DIPOLAR COUPLINGS OBTAINED BY TAKING THE DIFFERENCE IN THE J SPLITTINGS IN ISOTROPIC MEDIUM AND IN A LIQUID CRYSTALLINE MEDIUM (5% C12E5, R=0.96) (RUCKERT & OTTING (2000) J.AM.CHEM.SOC. 122, 7793-7797) MEASURED IN 2D IPAP (15N, 1H)-HSQC EXPERIMENTS (OTTIGER ET AL (1998) J.AM.CHEM.SOC. 131, 373-378) |
| 8 | 1 | 1 | INTERMOLECULAR DISTANCE RESTRAINTS OBTAINED FROM 12C-FILTERED/13C-SEPARATED AND 15N-SEPARATED NOE EXPERIMENTS ON COMPLEXES COMPRISING 1:1 AND 1:2 CVN:MAN-ALPHA-(1,2)-MAN-ALPHA |
| 9 | 1 | 1 | INTRA-DISACCHARIDE AND INTER-MANNOPYRANOSE DISTANCE RESTRAINTS OBTAINED FROM 12C-FILTERED NOE AND HOHAHA EXPERIMENTS ON COMPLEXES COMPRISING 1:1 AND 1:2 CVN:MAN-ALPHA-(1,2)-MAN-ALPHA. |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.4 | 300 |
Conformers
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 1 |
