1IIU
Chicken plasma retinol-binding protein (RBP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.064, 53.560, 73.414 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.280 - 2.500 |
R-factor | 0.21 * |
Rwork | 0.211 |
R-free | 0.26000 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 * |
RMSD bond angle | 2.800 * |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | AMoRE |
Refinement software | CNX (2000.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.120 * | 0.320 * |
Total number of observations | 22515 * | |
Number of reflections | 6664 * | 680 * |
Completeness [%] | 87.8 * | 68 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 * | 4 * | pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | MPD | 8 (%(v/v)) | |
3 | 1 | reservoir | cadmium acetate | 8 (mM) | |
4 | 1 | reservoir | sodium acetate | 20 (mM) | |
5 | 1 | reservoir | sodium cacodylate | 40 (mM) | pH6.6 |