1IIN
thymidylyltransferase complexed with UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 130 |
Detector technology | CCD |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.900, 112.141, 132.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.000* |
R-factor | 0.183 * |
Rwork | 0.184 |
R-free | 0.22300 * |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.740 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.830 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.075 * |
Completeness [%] | 99.7 * |
Redundancy | 7. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | Ammonium Sulfate, iso-propanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | HEPES | 10 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
6 | 1 | reservoir | isopropanol | 7.5 (%(v/v)) |