1IIM
thymidylyltransferase complexed with TTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 120.060, 120.060, 94.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.100 |
R-factor | 0.198 * |
Rwork | 0.198 |
R-free | 0.23500 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.260 |
High resolution limit [Å] | 2.010 |
Rmerge | 0.109 * |
Completeness [%] | 98.2 * |
Redundancy | 9. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 298 | Tris, Mono-ammonium dihydrogen phosphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | HEPES | 10 (mM) | |
5 | 1 | reservoir | ammonium phosphate | 2.0 (M) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
7 | 1 | reservoir | 20 (mM) |