1IID
Crystal Structure of Saccharomyces cerevisiae N-myristoyltransferase with Bound S-(2-oxo)pentadecylCoA and the Octapeptide GLYASKLA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-11 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.129, 97.060, 141.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.720 - 2.500 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nmt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.054 * | |
Total number of observations | 225123 * | |
Number of reflections | 17552 | |
Completeness [%] | 96.0 | 87.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 20 * | PEG 2000 MME, nickel chloride, sodium cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | reservoir | 10 (mM) | ||
3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.2 |
4 | 1 | reservoir | PEG2000 MME | 18 (%) |