1IGT
STRUCTURE OF IMMUNOGLOBULIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 291 |
Detector technology | AREA DETECTOR |
Collection date | 1992-11 |
Detector | XUONG-HAMLIN MULTIWIRE |
Spacegroup name | P 1 |
Unit cell lengths | 65.820, 76.770, 100.640 |
Unit cell angles | 88.05, 92.35, 97.23 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | VL:VH DOMAIN PAIR OF PDB ENTRY 1MCP CL:CH1 DOMAIN PAIR OF ENTRY 2HFL FC FRAGMENT OF ENTRY 1FC1 |
RMSD bond length | 0.009 |
RMSD bond angle | 27.100 * |
Data reduction software | HOWARD |
Data scaling software | XENGEN ((HOWARD) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 76.100 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.100 * |
Total number of observations | 200000 * |
Number of reflections | 47595 |
<I/σ(I)> | 7.1 |
Completeness [%] | 99.0 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | 18 * | Larson, S., (1991) J. Mol. Biol., 222, 17. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.1 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.01 (M) | |
3 | 1 | drop | PEG3350 | 1.8 (%) | |
4 | 1 | reservoir | PEG3350 | 4 (%) |