1IG9

Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	80.787, 118.602, 127.997
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 2.600
R-factor	0.197
Rwork	0.197
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1clq with non-protein atoms removed
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.086	0.610
Number of reflections	38733
<I/σ(I)>	15.4	2.7
Completeness [%]	99.4	99.8
Redundancy	5	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6 *	4 *	Wang, J., (1997) Cell (Cambridge,Mass.), 89, 1087. *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 350 monomethyl ether
2	1	1	calcium chloride
3	1	1	sodium cacodylate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	sodium citrate	0.05 (M)
3	1	drop	sodium potassium tartrate	0.025 (M)
4	1	drop	ammonium sulfate	0.5 (M)
5	1	reservoir	sodium citrate	0.1 (M)
6	1	reservoir	sodium potassium tartrate	0.5 (M)
7	1	reservoir	ammonium sulfate	1.0 (M)