1IFA
THREE-DIMENSIONAL CRYSTAL STRUCTURE OF RECOMBINANT MURINE INTERFERON-BETA
Experimental procedure
Spacegroup name | P 65 |
Unit cell lengths | 71.400, 71.400, 79.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 * - 2.600 |
R-factor | 0.205 |
Rwork | 0.205 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.059 * |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.067 * |
Total number of observations | 37143 * |
Number of reflections | 8045 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.3 * | 15 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | sodium azide | 0.02 (%) | |
3 | 1 | drop | dioxane | 14 (%) | 1.0 micro litter |
4 | 1 | reservoir | ammonium sulfate | 20 (%sat) | |
5 | 1 | reservoir | acetate | 50 (mM) |