1IFA

THREE-DIMENSIONAL CRYSTAL STRUCTURE OF RECOMBINANT MURINE INTERFERON-BETA

Experimental procedure

Spacegroup name	P 65
Unit cell lengths	71.400, 71.400, 79.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 * - 2.600
R-factor	0.205
Rwork	0.205
RMSD bond length	0.016
RMSD bond angle	0.059 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.067 *
Total number of observations	37143 *
Number of reflections	8045 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.3 *	15 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	sodium azide	0.02 (%)
3	1	drop	dioxane	14 (%)	1.0 micro litter
4	1	reservoir	ammonium sulfate	20 (%sat)
5	1	reservoir	acetate	50 (mM)