1IEB

HISTOCOMPATIBILITY ANTIGEN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 31 2 1
Unit cell lengths	77.530, 77.530, 319.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.222
Rwork	0.222
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iea
RMSD bond length	0.011
RMSD bond angle	26.300 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Rmerge	0.087 *
Total number of observations	85623 *
Number of reflections	29069
Completeness [%]	92.1
Redundancy	2.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.2		18% PEG 4000, 2% EG, 200MM A.S, 100MM CITRATE PH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5-10 (mg/ml)
2	1	2	PEG4000	16-18 (%)
3	1	2	ethylene glycol	2 (%)
4	1	2	ammonium sulfate	200 (mM)
5	1	2	citrate	100 (mM)