1ICW

INTERLEUKIN-8, MUTANT WITH GLU 38 REPLACED BY CYS AND CYS 50 REPLACED BY ALA

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1995-06-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	46.400, 49.200, 69.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.010
R-factor	0.194
Rwork	0.194
R-free	0.26600
RMSD bond length	0.010
RMSD bond angle	23.900 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.090
High resolution limit [Å]	2.010	2.010
Rmerge	0.043
Number of reflections	10544
<I/σ(I)>	27
Completeness [%]	96.0	78
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.2 *		protein solution is mixed in a 5:3 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	18 (mg/ml)
2	1	drop		250 (mM)
3	1	drop	MES	10 (mM)
4	1	reservoir	PEG3400	30 (%(w/v))
5	1	reservoir	ammonium sulfate	0.1 (M)