1ICF
CRYSTAL STRUCTURE OF MHC CLASS II ASSOCIATED P41 II FRAGMENT IN COMPLEX WITH CATHEPSIN L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.592, 80.594, 64.245 |
Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
Rwork | 0.182 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cjl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.380 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | MAIN |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.110 * |
Total number of observations | 132945 * |
Number of reflections | 42072 |
Completeness [%] | 97.0 |
Redundancy | 3.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 * | SITTING DROP VAPOR DIFFUSION METHOD RESERVOIR CONTAINED 1ML OF 0.2 M NA- ACETATE TRIHYDRATE, 30% W/V PEG 8K AND 0.1M MES, PH 6.1. DROP WAS COMPOSED OF 2 MICRO L OF RESERVOIR SOLUTION AND 2 MICRO L OF THE COMPLEX (10 MG/ML) IN 20MM NA-ACETATE AND 1MM EDTA, PH 5.0. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | Na acetate | 0.2 (M) | |
3 | 1 | reservoir | PEG8000 | 30 (%(v/v)) | |
4 | 1 | reservoir | MES | 0.1 (M) | |
5 | 1 | drop | Na acetate | 20 (mM) | |
6 | 1 | drop | EDTA | 1 (mM) |