1IC4

CRYSTAL STRUCTURE OF HYHEL-10 FV MUTANT(HD32A)-HEN LYSOZYME COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1998-03-16
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	57.219, 57.219, 237.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
Rwork	0.181
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.700
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.100	0.318 *
Total number of observations	74484 *
Number of reflections	17573
<I/σ(I)>	4.9
Completeness [%]	96.3	96.1 *
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.6	293	HEPES, MPD, PEG6000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	100 (mM)
2	1	1	PEG6000	9-11 (%(w/v))
3	1	1	MPD	7-9 (%(w/v))