1I8L
HUMAN B7-1/CTLA-4 CO-STIMULATORY COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.600, 183.238, 230.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.770 - 3.000 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HUMAN B7-1 DIMER |
RMSD bond length | 0.010 |
RMSD bond angle | 26.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.067 * | 0.351 * |
Total number of observations | 209381 * | |
Number of reflections | 38815 | 3636 * |
<I/σ(I)> | 19.1 | 3 |
Completeness [%] | 99.2 | 94.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 18 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | PEG8000 | 14 (%) | |
4 | 1 | reservoir | magnesium acetate | 200 (mM) | |
5 | 1 | reservoir | cacodylate | 100 (mM) |