1I7Q
ANTHRANILATE SYNTHASE FROM S. MARCESCENS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.316, 68.984, 116.363 |
| Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
| Resolution | 32.500 - 1.950 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIALLY REFINED MODEL |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.840 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0 REFMAC WARP) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.500 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.086 |
| Number of reflections | 88320 * |
| Completeness [%] | 92.0 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | pH 5.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 10 | 1 | reservoir | 8 (mM) | ||
| 11 | 1 | reservoir | tryptophan | 0.5 (mM) | |
| 2 | 1 | drop | glycerol | 15 (%(v/v)) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | EDTA | 10 (mM) | |
| 5 | 1 | drop | protein | 25 (mg/ml) | |
| 6 | 1 | reservoir | PEG6000 | 25 (%) | |
| 7 | 1 | reservoir | citrate | 100 (mM) | |
| 8 | 1 | reservoir | glutamate | 100 (mM) | |
| 9 | 1 | reservoir | glutamine | 34.5 (mM) |
