1I6L
1.7 HIGH RESOLUTION EXPERIMENTAL PHASES FOR TRYPTOPHANYL-TRNA SYNTHETASE COMPLEXED WITH TRYPTOPHANYL-5'AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-01 |
| Detector | BRANDEIS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.770, 59.770, 232.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.720 |
| R-factor | 0.2 * |
| Rwork | 0.209 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.409 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.078 * | 0.571 * |
| Total number of observations | 1045314 * | |
| Number of reflections | 51522 | |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 95.7 | 68.8 |
| Redundancy | 5.15 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.6 | 310 | POTASSIUM PHOSPHATE, AMMONIUM SULFATE, MAGNESIUM SULFATE, pH 7.60, MICRODIALYSIS, temperature 310K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | tryptophan | 2 (mM) | |
| 2 | 1 | 1 | ATP | 10 (mM) | |
| 3 | 1 | 1 | magnesium | 10 (mM) | |
| 4 | 1 | 2 | potassium phosphate | 2.0 (M) |
