1I2P
CRYSTAL STRUCTURE OF ESCHERICHIA COLI TRANSALDOLASE B MUTANT D17A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-29 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.381, 92.788, 131.537 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.960 - 2.050 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.22100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1onr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.094 | 0.344 |
Total number of observations | 458342 * | |
Number of reflections | 49932 | |
<I/σ(I)> | 13 | 2.1 |
Completeness [%] | 92.4 | 85.4 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | used microseeding, Jia, J., (1996) Acta Crystallogr., Sect.D, 52, 192. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 11-13 (%(w/w)) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) |