1I1R

CRYSTAL STRUCTURE OF A CYTOKINE/RECEPTOR COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-21
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	103.000, 123.310, 76.790
Unit cell angles	90.00, 112.03, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.213
Rwork	0.213
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human Interleukin-6 1ALU gp130 D2D3 domains 1BQU
RMSD bond length	0.007
RMSD bond angle	1.600
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.081	0.487
Total number of observations	106397 *
Number of reflections	34376
<I/σ(I)>	3.4	1.4
Completeness [%]	99.0	99.6
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	293	MPEG 2000, sodium citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	mPEG2000	10 (%)
3	1	1	Na citrate	0.1 (M)