1I1I

NEUROLYSIN (ENDOPEPTIDASE 24.16) CRYSTAL STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	108
Detector technology	CCD
Collection date	1999-07-12
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	157.600, 87.700, 58.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
Rwork	0.224
R-free	0.26800
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	20.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.042	0.156
Number of reflections	36511
<I/σ(I)>	23.9	7.8
Completeness [%]	99.0	95.5
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	277	PEG 8000, cacodylate, magnesium chloride, zinc chloride, 2-mercaptoethanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		0.2 (mM)