1I1C

NON-FCRN BINDING FC FRAGMENT OF RAT IGG2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-08-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	42.503, 73.362, 82.157
Unit cell angles	90.00, 102.74, 90.00

Refinement procedure

Resolution	29.810 - 2.700
R-factor	0.241 *
Rwork	0.241
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fc1
RMSD bond length	0.008 *
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.068	0.261 *
Total number of observations	35621 *
Number of reflections	14919 *	1323 *
<I/σ(I)>	13.4	3.6
Completeness [%]	97.5 *	87.2
Redundancy	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6 *	298	PEG 4000 ammonium acetate tri-sodium acetate dihydrate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	0.2 (M)
2	1	reservoir	trisodium acetate dihydrate	0.1 (M)
3	1	reservoir	PEG4000	30 (%)