1I0I
ANALYSIS OF AN INVARIANT ASPARTIC ACID IN HPRTS-GLUTAMINE MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-25 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.380, 101.270, 51.670 |
| Unit cell angles | 90.00, 94.25, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.060 |
| R-factor | 0.168 * |
| Rwork | 0.168 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc2 with ligands waters and loop II removed |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.068 | 0.411 |
| Number of reflections | 21963 * | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 92.1 | 56.2 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 6000, Sodium acetate, Ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP |
