1I0C
EPS8 SH3 CLOSED MONOMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | P 31 |
| Unit cell lengths | 49.736, 49.736, 36.461 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.000 - 2.000 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.900 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.540 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.069 |
| Total number of observations | 24299 * |
| Number of reflections | 6795 |
| Completeness [%] | 99.7 * |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 | pH 4.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 21 (%) | |
| 2 | 1 | reservoir | isopropanol | 10 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 4 | 1 | drop | protein | 14 (mg/ml) |
