1HZJ
HUMAN UDP-GALACTOSE 4-EPIMERASE: ACCOMMODATION OF UDP-N-ACETYLGLUCOSAMINE WITHIN THE ACTIVE SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-24 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.400, 88.900, 118.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.184 * |
| Rwork | 0.184 |
| R-free | 0.22700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ek6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.100 |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | 0.286 |
| Number of reflections | 104972 | 8999 * |
| <I/σ(I)> | 26.8 | 2.9 |
| Completeness [%] | 97.0 | 84.6 |
| Redundancy | 5.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 4 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MES | 100 (mM) | |
| 2 | 1 | 1 | PEG3400 | 8-9 (%(w/v)) | |
| 3 | 1 | 1 | 75 (mM) |
