1HZF
C4ADG FRAGMENT OF HUMAN COMPLEMENT FACTOR C4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.510, 71.430, 85.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 2.300 |
| Rwork | 0.215 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.040 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.840 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.079 | 0.511 |
| Total number of observations | 43751 * | |
| Number of reflections | 15703 | 1057 * |
| <I/σ(I)> | 13.6 | 2 |
| Completeness [%] | 97.9 | 99.4 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 298 | PEG 4000, TRIS, magnesium chloride, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 28 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 10 (mM) | pH8.5 |
| 7 | 1 | drop | 10 (mM) | ||
| 8 | 1 | drop | EDTA | 2 (mM) | |
| 9 | 1 | drop | dithiothreitol | 5 (mM) |
