1HYU
CRYSTAL STRUCTURE OF INTACT AHPF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1998-08-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.923,0.97980,0.97961,0.96482 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.200, 139.160, 39.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.000 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 24.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.357 |
| Number of reflections | 38368 | |
| <I/σ(I)> | 12.6 | 5.1 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 4 * | 1.7M Ammonium sulphate, 1.0M Lithium Chloride, 0.1M citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 3 | 1 | reservoir | lithium chloride | 1.0 (M) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) |
