1HYE
CRYSTAL STRUCTURE OF THE MJ0490 GENE PRODUCT, THE FAMILY OF LACTATE/MALATE DEHYDROGENASE, DIMERIC STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.647, 125.101, 58.082 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.490 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22800 |
| Starting model (for MR) | 1a5z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.393 |
| Total number of observations | 113866 * | |
| Number of reflections | 26843 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 95.2 | 80 |
| Redundancy | 2.8 | 1103 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 287 * | 2-methyl-2,4-pentanediol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | PMSF | 1.0 (mM) | |
| 6 | 1 | reservoir | detergent | 18 (mM) | |
| 7 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
| 8 | 1 | reservoir | mPEG350 | 10 (%) | |
| 9 | 1 | reservoir | PEG400 | 12 (%) |
