1HWY
BOVINE GLUTAMATE DEHYDROGENASE COMPLEXED WITH NAD AND 2-OXOGLUTARATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 122.500, 101.000, 164.600 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.200 |
| Rwork | 0.230 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.400 |
| High resolution limit [Å] | 3.000 | 3.200 |
| Rmerge | 0.123 * | 0.475 * |
| Completeness [%] | 79.0 * | 80 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | sodium phosphate, NaCl, PEG 8000, sodium azide, methyl pentanediol, octyl-b-glucopyranoside, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | NAD+ | 1.5 (mM) | |
| 2 | 1 | drop | alpha-KG | 20 (mM) | |
| 3 | 1 | drop | boGDH | 3.25 (mg/ml) | |
| 4 | 1 | reservoir | sodium phosphate | 0.1 (M) | |
| 5 | 1 | reservoir | octyl-beta-glucopyranoside | 1 (%) | |
| 6 | 1 | reservoir | methyl pentanediol | 5 (%) | |
| 7 | 1 | reservoir | sodium chloride | 0.5 (M) | |
| 8 | 1 | reservoir | sodium azide | 1 (mM) |
