1HWW
GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54189 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.902, 110.015, 138.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 * - 1.870 |
R-factor | 0.181 * |
Rwork | 0.181 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hty |
RMSD bond length | 0.005 |
RMSD bond angle | 1.310 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.240 | 1.910 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.078 | 0.452 |
Number of reflections | 87386 * | 5601 * |
<I/σ(I)> | 24 | 3.8 |
Completeness [%] | 99.7 | 96.9 |
Redundancy | 6.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 298 | PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 8.5 (%) | |
2 | 1 | reservoir | MPD | 5 (%) | |
3 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |