1HWL
COMPLEX OF THE CATALYTIC PORTION OF HUMAN HMG-COA REDUCTASE WITH ROSUVASTATIN (FORMALLY KNOWN AS ZD4522)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2000-05-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.432, 172.512, 79.987 |
Unit cell angles | 90.00, 117.36, 90.00 |
Refinement procedure
Resolution | 43.290 - 2.100 |
R-factor | 0.219 * |
Rwork | 0.219 |
R-free | 0.23900 |
Structure solution method | difference fourier |
Starting model (for MR) | 1dqa |
RMSD bond length | 0.087 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.072 | 0.473 |
Number of reflections | 101858 | |
<I/σ(I)> | 21.1 | 1.3 |
Completeness [%] | 97.6 | 87.6 |
Redundancy | 5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.5 | 21 * | used microseeding * |
1 | Batch method * | 7.5 | 21 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 3-5 (mg/ml) | |
2 | 1 | 1 | PEG4000 | 12-15 (%(w/v)) | |
3 | 1 | 1 | ammonium acetate | 0.15-0.2 (M) | |
4 | 1 | 1 | sodium HEPES | 25 (mM) | |
5 | 1 | 1 | dithiothreitol | 50 (mM) | |
6 | 1 | 1 | ADP | 10 (mM) | |
7 | 1 | 1 | glycerol | 10 (%) |