1HW1
THE FADR-DNA COMPLEX: TRANSCRIPTIONAL CONTROL OF FATTY ACID METABOLISM IN ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-02 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.346, 86.996, 59.128 |
Unit cell angles | 90.00, 120.18, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.500 |
R-factor | 0.198 * |
Rwork | 0.198 |
R-free | 0.21600 |
Structure solution method | MAD |
RMSD bond length | 0.004 * |
RMSD bond angle | 1.109 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 1.500 | |
Rmerge | 0.067 | 0.180 * |
Total number of observations | 1306223 * | |
Number of reflections | 80658 | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 97.2 | 78.9 * |
Redundancy | 16.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | 18 * | ammonium sulfate, lithium sulfate, sodium citrate, zinc chloride, pH 5.6, EVAPORATION, temperature 290.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
3 | 1 | reservoir | ammonium sulfate | 0.7 (M) | |
4 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
5 | 1 | reservoir | zinc chloride | 5 (mM) |