1HVU
HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 REVERSE TRANSCRIPTASE COMPLEXED WITH A 33-BASE NUCLEOTIDE RNA PSEUDOKNOT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1995-03-15 |
| Detector | CUSTOM-MADE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.700, 169.200, 331.400 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 4.750 |
| R-factor | 0.34 |
| Rwork | 0.340 |
| R-free | 0.41300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3HVT AND 1HMV |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.853) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 4.750 | |
| Rmerge | 0.064 * | 0.263 * |
| Number of reflections | 32343 | |
| Completeness [%] | 81.0 | 56.9 * |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 2 | 1 | 1 | HEXA-B-D-GLYCOPYRANOSIDE | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 1 | GLYCEROL | ||
| 5 | 1 | 1 | NAN3 | ||
| 6 | 1 | 1 | DIOXANE | ||
| 7 | 1 | 2 | SODIUM CITRATE | ||
| 8 | 1 | 2 | MAGNESIUM ACETATE | ||
| 9 | 1 | 2 | IOXANE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | citrate | 1.0 (M) | |
| 2 | 1 | reservoir | bis-Tris-propane | 50 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 100 (mM) |
