1HVC

CRYSTAL STRUCTURE OF A TETHERED DIMER OF HIV-1 PROTEASE COMPLEXED WITH AN INHIBITOR

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	52.100, 59.800, 62.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.2 *
RMSD bond length	0.018
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.064 *
Total number of observations	39662 *
Number of reflections	16421 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HIV PR	6 (mg/ml)
10	1	reservoir		132 (mM)
11	1	reservoir	ammonium sulfate	34 (%sat)
2	1	drop	A-76928
3	1	drop	sodium acetate	50 (mM)
4	1	drop	DTT	10 (mM)
5	1	drop	DMSO	10 (%)
6	1	drop	sodium citrate	66 (mM)
7	1	drop		132 (mM)
8	1	drop	ammonium sulfate	34 (%sat)
9	1	reservoir	sodium citrate	66 (mM)