1HUW

THE CRYSTAL STRUCTURE OF AFFINITY-MATURED HUMAN GROWTH HORMONE AT 2 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	80.450, 59.340, 50.870
Unit cell angles	90.00, 128.20, 90.00

Refinement procedure

Resolution	8.000 - 2.400 *
R-factor	0.185
Rwork	0.185
RMSD bond length	0.012
RMSD bond angle	2.630
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.069 *	0.144 *
Total number of observations	16335 *
Number of reflections	6630 *	626 *
Completeness [%]	97.3 *	94.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%(w/v))
10	1	drop	magnesium acetate	0.2 (M)
11	1	drop	sodium cacodylate	0.1 (M)
2	1	reservoir	magnesium acetate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)
4	1	drop	protein	6 (mg/ml)
5	1	drop		120 (mM)
6	1	drop	sodium cacoylate	20 (mM)
7	1	drop	EDTA	5.0 (mM)
8	1	drop	phenylmethylsulfonyl fluoride	1 (mM)
9	1	drop	PEG8000	10 (%(w/v))