1HQM

CRYSTAL STRUCTURE OF THERMUS AQUATICUS CORE RNA POLYMERASE-INCLUDES COMPLETE STRUCTURE WITH SIDE-CHAINS (EXCEPT FOR DISORDERED REGIONS)-FURTHER REFINED FROM ORIGINAL DEPOSITION-CONTAINS ADDITIONAL SEQUENCE INFORMATION

Replaces:  1DDQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-12-10
Detector	ADSC QUANTUM 4
Wavelength(s)	.9372
Spacegroup name	P 41 21 2
Unit cell lengths	200.760, 200.760, 292.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.300
R-factor	0.28 *
Rwork	0.300
R-free	0.36000
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.690
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.100	0.455
Number of reflections	102412
<I/σ(I)>	4.7
Completeness [%]	82.5	50.9
Redundancy	2.3	0.51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	Zhang, G., (1999) Cell, 98, 811. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	ammonium sulfate	40-45 (%sat)
3	1		Tris-HCl	0.1 (M)