1HQC
STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.885 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.865, 84.865, 355.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.200 |
| R-factor | 0.263 * |
| Rwork | 0.263 |
| R-free | 0.29650 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.530 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 12.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.121 |
| Number of reflections | 21630 |
| <I/σ(I)> | 8.36 |
| Completeness [%] | 99.6 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG 4000, sodium chloride, magnesium chloride, ADP, AMPPNP, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | 0.35 (M) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | PEG4000 | 1 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 6 | 1 | reservoir | 2.0 (M) | ||
| 7 | 1 | reservoir | glycerol | 10 (%) | |
| 8 | 1 | reservoir | PEG4000 | 10 (%) |
