1HQC
STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-28 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.885 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.865, 84.865, 355.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 3.200 |
R-factor | 0.263 * |
Rwork | 0.263 |
R-free | 0.29650 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.530 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 12.000 |
High resolution limit [Å] | 3.200 |
Rmerge | 0.121 |
Number of reflections | 21630 |
<I/σ(I)> | 8.36 |
Completeness [%] | 99.6 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG 4000, sodium chloride, magnesium chloride, ADP, AMPPNP, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | |
2 | 1 | drop | 0.35 (M) | ||
3 | 1 | drop | glycerol | 10 (%) | |
4 | 1 | drop | PEG4000 | 1 (%) | |
5 | 1 | reservoir | Tris-HCl | 20 (mM) | |
6 | 1 | reservoir | 2.0 (M) | ||
7 | 1 | reservoir | glycerol | 10 (%) | |
8 | 1 | reservoir | PEG4000 | 10 (%) |