1HPL
HORSE PANCREATIC LIPASE. THE CRYSTAL STRUCTURE AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.800, 97.200, 145.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.300 |
R-factor | 0.159 |
Rwork | 0.159 |
RMSD bond length | 0.015 |
RMSD bond angle | 3.100 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.300 * | 2.300 * |
Rmerge | 0.063 * | |
Total number of observations | 145844 * | |
Number of reflections | 45644 * | |
Completeness [%] | 86.3 * | 48 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 * | 20 * | referred to J.Mol.Biol. 205.259-261 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 8 (mg/ml) | |
2 | 1 | 2 | MES | 0.1 (M) | |
3 | 1 | 2 | PEG8000 | 15-20 (%(w/v)) | |
4 | 1 | 2 | 10 (mM) | ||
5 | 1 | 2 | 0.1 (M) |