1HOZ

CRYSTAL STRUCTURE OF AN INOSINE-ADENOSINE-GUANOSINE-PREFERRING NUCLEOSIDE HYDROLASE FROM TRYPANOSOMA VIVAX

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-11-10
Detector	MARRESEARCH
Wavelength(s)	0.8445
Spacegroup name	P 1 21 1
Unit cell lengths	52.090, 74.000, 82.000
Unit cell angles	90.00, 104.56, 90.00

Refinement procedure

Resolution	30.000 - 1.600
R-factor	0.1861 *
Rwork	0.186
R-free	0.20960 *
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.560
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.055	0.385 *
Total number of observations	629834 *
Number of reflections	629834
<I/σ(I)>	10.4	2
Completeness [%]	99.8	99.5
Redundancy	4.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM tris, 1.6 M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	Tris	100 (mM)	pH8.5
3	1	reservoir	ammonium sulfate	1.6 (M)