1HOZ
CRYSTAL STRUCTURE OF AN INOSINE-ADENOSINE-GUANOSINE-PREFERRING NUCLEOSIDE HYDROLASE FROM TRYPANOSOMA VIVAX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8445 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.090, 74.000, 82.000 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.1861 * |
| Rwork | 0.186 |
| R-free | 0.20960 * |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.560 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.385 * |
| Total number of observations | 629834 * | |
| Number of reflections | 629834 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM tris, 1.6 M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 3 | 1 | reservoir | ammonium sulfate | 1.6 (M) |
