1HM5
CRYSTAL STRUCTURE ANALYSIS OF THE RABBIT D-GLUCOSE 6-PHOSPHATE ISOMERASE (NO LIGAND BOUND)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.756, 115.966, 271.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.21200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.258 |
| Total number of observations | 398627 * | |
| Number of reflections | 105425 | |
| <I/σ(I)> | 25.4 | 3.72 |
| Completeness [%] | 87.2 | 47.5 |
| Redundancy | 3.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | 14% PEG 8000, 0.1M sodium cacodylate, 0.25M magnesium chloride, pH 6.6 mixed 1:1 with protein in solution 10mM imidazole, 50mM potassium chloride, pH7.5, VAPOR DIFFUSION, HANGING DROP at 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | imidazole | 10 (mM) | pH7.5 |
| 4 | 1 | reservoir | PEG8000 | 12-14 (%(w/v)) | |
| 5 | 1 | reservoir | magnesium acetate | 250 (mM) | |
| 6 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
