1HLZ
CRYSTAL STRUCTURE OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB(ALPHA) DNA-BINDING DOMAIN BOUND TO ITS COGNATE RESPONSE ELEMENT
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-05 |
Detector | FUJI |
Wavelength(s) | 0.92016 |
Spacegroup name | P 1 |
Unit cell lengths | 35.867, 39.603, 58.565 |
Unit cell angles | 84.13, 72.09, 70.20 |
Refinement procedure
Resolution | 18.600 - 2.800 |
R-factor | 0.2599 |
Rwork | 0.260 |
R-free | 0.31030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a6y residues 132-198 from chain A & B plus DNA |
RMSD bond length | 0.009 * |
RMSD bond angle | 2.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.620 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.078 * | 0.258 * |
Number of reflections | 6854 * | 732 * |
<I/σ(I)> | 9 | 2.1 |
Completeness [%] | 96.6 * | 93.3 * |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 17 * | Zhao, Q., (1998) Mol. Cell, 1, 849. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG8000 | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | Tris |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 0.6 (mM) | |
2 | 1 | drop | protein | 1.2 (mM) | |
3 | 1 | reservoir | PEG8000 | 20-25 (%) | |
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | 400 (mM) | ||
6 | 1 | reservoir | Tris | pH7.5 |