1HLU

STRUCTURE OF BOVINE BETA-ACTIN-PROFILIN COMPLEX WITH ACTIN BOUND ATP PHOSPHATES SOLVENT ACCESSIBLE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	288
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1995-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	38.140, 72.240, 185.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.650
R-factor	0.201
Rwork	0.201
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2btf
RMSD bond length	0.015
RMSD bond angle	2.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.082	0.267
Number of reflections	13869	533 *
<I/σ(I)>	13.5	2.4
Completeness [%]	87.3	69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7.5		Rozycki, M.D., (1991) Methods Enzymol., 196, 100. *