1HLE

CRYSTAL STRUCTURE OF CLEAVED EQUINE LEUCOCYTE ELASTASE INHIBITOR DETERMINED AT 1.95 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	121.370, 103.570, 80.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.950
R-factor	0.176
Rwork	0.176
RMSD bond length	0.015
RMSD bond angle	1.570
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.950 *	1.950 *
Rmerge	0.096 *
Total number of observations	154128 *
Number of reflections	38335 *
Completeness [%]	98.7 *	97.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	MOPS	5 (mM)
3	1	reservoir	PEG4000	16-18 (%(w/v))
4	1	reservoir		0.18 (M)