1HKU

CtBP/BARS: a dual-function protein involved in transcription corepression and Golgi membrane fission

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 64 2 2
Unit cell lengths	88.705, 88.705, 163.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.222
Rwork	0.222
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.059	0.302 *
Number of reflections	17070
<I/σ(I)>	25.5	8.7
Completeness [%]	99.3	100
Redundancy	11.0 *	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		2.0 M AMMONIUM FORMATE, 100 MM HEPES, PH 7.5.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium formate	1.8-2.1 (M)
2	1	reservoir	HEPES	100 (mM)	pH7.5