1HKN
A complex between acidic fibroblast growth factor and 5-amino-2-naphthalenesulfonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 96.530, 47.210, 97.840 |
| Unit cell angles | 90.00, 107.04, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : PDB ENTRY 2AXM |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.230 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.500 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.219 |
| Total number of observations | 321819 * | |
| Number of reflections | 56957 | |
| <I/σ(I)> | 9.7 | 2.4 |
| Completeness [%] | 99.1 | 99.1 * |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.85 * | 295 * | CRYSTALS OF THE COMPLEX BETWEEN AFGF AND 5-AMINO-2-NMS WERE GROWN BY MIXING 0.75 MM PROTEIN, 1.5 MM OF THE INHIBITOR AND 60% SODIUM/PO, pH 7.80 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.75 (mM) | |
| 2 | 1 | drop | inhibitor | 1.5 (mM) | |
| 3 | 1 | drop | sodium potassium tartrate | 60 (%) | |
| 4 | 1 | drop | sodium phosphate | 5 (mM) | pH7.85 |
| 5 | 1 | reservoir | 1.3 (M) |
