1HJX
Ligand-induced signalling and conformational change of the 39 kD glycoprotein from human articular chondrocytes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2002-09-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.562, 122.048, 136.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 1.850 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lg2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.076 | 0.519 |
Number of reflections | 153614 | |
<I/σ(I)> | 9.5 | 3.7 |
Completeness [%] | 99.0 | 98.4 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 4.6, 25% PEG 4000, 100 MM DTT |