1HJR
ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.800, 139.600, 32.400 |
| Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.039 * |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Number of reflections | 15545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | Ariyoshi, M., (1994) J.Mol.Biol., 241, 281. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein solution | 8.0 (mg/ml) | |
| 2 | 1 | 2 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 2 | 300-400 (mM) | ||
| 4 | 1 | 2 | glycerol | 7.5 (%(v/v)) | |
| 5 | 1 | 2 | EDTA | 1 (mM) | |
| 6 | 1 | 2 | DTT | 1 (mM) |
