1HJB
CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN AND C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.165, 109.273, 127.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1io4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 20.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.006 * | |
| Total number of observations | 117670 * | |
| Number of reflections | 26407 | |
| <I/σ(I)> | 13.1 | 1.4 |
| Completeness [%] | 91.3 | 71.9 |
| Redundancy | 4.456 | 1.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 297 | Tahirov, T.H., (2001) Acta Crystallogr., D57, 850. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA solution | 10 (mg/ml) | |
| 2 | 1 | reservoir | 0.1 (M) | ||
| 3 | 1 | reservoir | 0.01 (M) | ||
| 4 | 1 | reservoir | PEG400 | 10 (%(v/v)) | |
| 5 | 1 | reservoir | MES | 0.05 (M) |
