1HIG

THREE-DIMENSIONAL STRUCTURE OF RECOMBINANT HUMAN INTERFERON-GAMMA.

Experimental procedure

Spacegroup name	H 3 2
Unit cell lengths	114.000, 114.000, 315.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.800 *
R-factor	0.25 *
Rwork	0.250
RMSD bond length	0.022
RMSD bond angle	4.740
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.113 *
Total number of observations	158195 *
Number of reflections	19954 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.9 *	25 *	referred to J.Biol.Chem. 262.4804 1987 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.0025ml
2	1	drop	ammonium sulfate	36 (%sat)	0.0025ml
3	1	drop	acetate	50 (mM)
4	1	reservoir	ammonium sulfate	36 (%sat)
5	1	reservoir	acetate	50 (mM)