1HI0
RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus initiation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.650, 92.700, 140.790 |
Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TBA |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.170 | 0.960 |
Number of reflections | 56000 | |
<I/σ(I)> | 7.1 | 1.1 |
Completeness [%] | 95.0 | 86 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.7 | 293 * | Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, 56, 1473. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 10 (%) | |
3 | 1 | reservoir | MES | 0.1 (M) |